Year: 2020 | Month: October | Volume 10 | Issue 5
Incidence of Colibacillosis in Poultry in Gonda District in North Eastern Plain Zone of Uttar Pradesh
Abhishek Mishra
Debasish Niyogi
Rakesh Kumar Gupta
Krishna Kant Tripathi
Satya Vrat Singh
DOI:10.30954/2277-940X.05.2020.23
Abstract:
The present study was undertaken with the objective to study the incidence of E. coli infection in broiler chicken in Gonda district of Uttar Pradesh. For this study a total number of 483 naturally dead broiler chickens from 8 different private farms of Gonda district were collected during June 2018 – May 2019. The overall incidence of colibacillosis was 38.09% (184 samples were positive out of 483 samples). The gross change of liver was found as enlarged, congested and covered with thick yellow/white serofibrinous covering. Heart was also covered with thick yellow/white serofibrinous covering. Among tested 483 samples from 8 private farms from Gonda district, 184 (38.09%) were found to be suggestive of E. coli giving typical large (2-3 mm) lactose fermenting pink coloured colonies on Mac Conkey’s agar plate. They again produced characteristic ‘metallic sheen’ on EMB agar. On Gram staining during morphological study, they produced pink coloured, rod shaped ones (that is gram negative) with characteristic arrangement. All these isolates (from district Gonda) showed typical biochemical reaction which were positive to Indole test, M.R. test, T.S.I. agar test, nitrate reduction test and negative to VP test and H2S production test. Among 184 E. coli isolates 98.36% were negative to citrate utilization test and 97.82% to urease activity test. Out of 184 isolates of E. coli 179 were motile. They all fermented D-glucose, lactose, manitol. Among 184 isolates of E. coli, 154 (83.69%) isolates, 158(85.86%) isolates and 144 (78.26%) isolates had shown fermentation of sucrose, dulcitol and salicin respectively.
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